Activation of formylmethanofurna synthesis in cell extracts of Methanobacterium thermoautotrophicum

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Journal of Bacteriology; (USA)

OSTI ID:7147023

Bobik, T A; Wolfe, R S ^[1]

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1. Univ. of Illinois, Urbana (USA)

In cell extracts of Methanobacterium thermoautotrophicum, formylmethanofuran (formyl-MFR) synthesis (an essential CO{sub 2} fixation reaction that is an early step in CO{sub 2} reduction to methane) is subject to a complex activation that involves a heterodisulfide of coenzyme M and N-(7-mercaptoheptanoyl)threonine O{sup 3}-phosphate (CoM-S-S-HTP). In this paper we report that titanium(III) citrate, a low-potential reducing agent, stimulated CO{sub 2} reduction to methane and activated formyl-MFR synthesis in cell extracts. Titanium(III) citrate functioned as the sole source of electrons for formyl-MFR synthesis and enabled this reaction to occur independently of CoM-S-S-HTP. In addition, CoM-S-S-HTP was found to activate an unknown electron carrier that reduced metronidazole. The activation of formyl-MFR synthesis by CoM-S-S-HTP may involve the activation of a low-potential electron carrier.

• OSTI ID: 7147023
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:3; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALKANES
BACTERIA
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
COENZYMES
ELECTRONS
ELEMENTARY PARTICLES
FERMIONS
GROWTH
HYDROCARBONS
LEPTONS
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
REAGENTS
SYNTHESIS
TITANIUM COMPOUNDS
TRANSITION ELEMENT COMPOUNDS