Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Molecular cloning and amino acid sequence of leukotriene A/sub 4/ hydrolase

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ; ; ; ;

A cDNA clone corresponding to leukotriene A/sub 4/ hydrolase was isolated from a human lung lambdagt11 expression library by immunoscreening with a polyclonal antiserum. Several additional clones from human lung and placenta cDNA lambdagt11 libraries were obtained by plaque hybridization with the /sup 32/P-labeled lung cDNA clone. One of these clones has an insert of 1910 base pairs that contains the complete protein-coding region. From the deduced primary structure, leukotriene A/sub 4/ hydrolase is a 610 amino acid protein with a calculated molecular weight of 69,140. No apparent homologies with microsomal epoxide hydrolases were found. RNA blot analysis indicated substantial amounts of a discrete mRNA of approx. 2250 nucleotides in lung tissue and leukocytes.

Research Organization:
Karolinska Institutet, Stockholm (Sweden)
OSTI ID:
7143529
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:19; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Molecular cloning and amino acid sequence of human 5-lipoxygenase
Journal Article · Thu Dec 31 23:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:6464728
Cloning of a cDNA encoding a surface antigen of Schistosoma mansoni schistosomula recognized by sera of vassinated mice
Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5297134
cDNA cloning of human myeloperoxidase: decrease in myeloperoxidase mRNA upon induction of HL-60 cells
Journal Article · Tue Mar 31 23:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5903627

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BACTERIOPHAGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CLONING
DAYS LIVING RADIOISOTOPES
DNA
DNA-CLONING
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
FETAL MEMBRANES
HYBRIDIZATION
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
LUNGS
MAMMALS
MAN
MATERIALS
MEMBRANES
MESSENGER-RNA
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASITES
PLACENTA
PRIMATES
RADIOISOTOPES
RECOMBINANT DNA
RESPIRATORY SYSTEM
RNA
SULFUR 35
SULFUR ISOTOPES
VERTEBRATES
VIRUSES