Effects of preventing O-glycosylation on the secretion of human chorionic gonadotropin in Chinese hamster ovary cells

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.18.6354· OSTI ID:7143415

Matzuk, M M; Krieger, M; Corless, C L; Boime, I

Human chorionic gonadotropin (hCG) is a member of a family of heterodimeric glycoprotein hormones that have a common ..cap alpha.. subunit but differ in their hormone-specific ..beta..-subunits. The ..beta.. subunit of hCG (hCG..beta..) is unique among the ..beta.. subunits in that it contains four mucin-like O-linked oligosaccharides attached to a carboxyl-terminal extension. To study the effects of O-glycosylation on the secretion and assembly of hCG, expression vectors containing either hCG..beta.. gene alone or together with the hCG..cap alpha.. gene were transfected into a mutant Chinese hamster ovary cell line, 1d1D, which exhibits a reversible defect in O-glycosylation. The results reveal that hCG..beta.. can be secreted normally in the absence of its O-linked oligosaccharides. hCG..beta.. devoid of O-linked carbohydrate can also combine efficiently with hCG..cap alpha.. and be secreted as an intact dimer. The authors conclude that in Chinese hamster ovary cells, the hCG..beta.. O-linked chains play no role in the assembly and secretion of hCG. The normal and O-linked oligosaccharide-deficient forms of hCG secreted by these cells should prove useful in examining the role of O-linked chains on the biological function of hCG.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO (USA)
• OSTI ID: 7143415
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:18
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOPROTEINS
ENZYMATIC HYDROLYSIS
HCG
POST-TRANSLATION MODIFICATION
SECRETION
CHO CELLS
CYSTEINE
ELECTROPHORESIS
GENE RECOMBINATION
GENES
IMMUNE REACTIONS
O-GLYCOSYL HYDROLASES
OLIGONUCLEOTIDES
SULFUR 35
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ENZYMES
EVEN-ODD NUCLEI
GLYCOSYL HYDROLASES
GONADOTROPINS
HORMONES
HYDROLASES
HYDROLYSIS
ISOTOPES
LIGHT NUCLEI
LYSIS
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
SACCHARIDES
SOLVOLYSIS
SULFUR ISOTOPES
THIOLS
550201* - Biochemistry- Tracer Techniques