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Trans-activation of human immunodeficiency virus gene expression is mediated by nuclear events

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;
Human immunodeficiency virus encodes a gene product termed tat that is able to activate viral gene expression when present in trans. The mechanism of action of the tat gene product appears to be bimodal, resulting in both an increase in the steady-state level of viral mRNA and the enhanced translation of that RNA. In this report, the authors have examined the mechanism by which tat elevates viral mRNA levels. Data are presented demonstrating that tat acts by increasing the rate of viral transcription, rather than by modulating the stability of viral mRNA. Indirect immunofluorescence was used to show that tat is predominantly localized in the nucleus of expressing cells, a location consistent with a role in the regulation of viral transcription. These results suggest that tat could play a role in human immunodeficiency virus replication essentially similar to that proposed for the trans-acting nuclear gene products described for several other virus species.
Research Organization:
Roche Research Center, Nutley, NJ (USA)
OSTI ID:
7143406
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:18; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AIDS VIRUS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CELL CONSTITUENTS
CELL NUCLEI
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DNA
FLUORESCENCE
GENE REGULATION
GENES
GROWTH FACTORS
INTERFERON
ISOTOPES
LIGHT NUCLEI
LUMINESCENCE
LYMPHOKINES
MESSENGER-RNA
MICROORGANISMS
MITOGENS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RNA
TRANSCRIPTION
VIRUSES