Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Isolation and characterization of three endosomal fractions from the liver of estradiol-treated rats

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ; ; ; ;

Three distinct endosomal fractions were isolated in high purity from livers of estradiol-treated rats. Each fraction had characteristic physical and ultrastructural properties, but the lipid composition and major proteins of their membranes were similar and differed from those derived from the Golgi apparatus. Injected radioiodinated low density lipoproteins accumulated first in the fraction of intermediate density and later in the low density fraction. The latter was composed almost exclusively of lipoprotein-filled multivesicular bodies, most of which had a single membranous appendage. The fraction of intermediate density was composed of lipoprotein-filled vesicles that were smaller than multivesicular bodies and also had membranous appendages. The high density fraction was composed of membranes resembling the appendages of the two vesicular fractions. All three fractions were enriched in receptors for low density lipoproteins and asialoglycoproteins, but receptor concentrations were considerably reduced in multivesicular bodies. The fraction of intermediate density may represent the compartment of uncoupling of receptor and ligand (CURL) described by Geuze et al. CURL vesicles may lose some of their appendages as multivesicular bodies are formed. The high density fraction than may represent a receptor-recycling compartment.

Research Organization:
Univ. of California, San Francisco (USA)
OSTI ID:
7143293
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:19; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Lipoprotein binding and endosomal itinerary of the low density lipoprotein receptor-related protein in rat liver
Journal Article · Thu Nov 30 23:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA) · OSTI ID:6921652
The small GTP-binding protein rab4 is associated with early endosomes
Journal Article · Mon Jul 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:6096682
78 kDa receptor for Man6P-independent lysosomal enzyme targeting: Biosynthetic transport from endoplasmic reticulum to 'high-density vesicles'
Journal Article · Sat Apr 15 00:00:00 EDT 2006 · Experimental Cell Research · OSTI ID:20775359

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENZYME ACTIVITY
ESTRADIOL
ESTRANES
ESTROGENS
GLANDS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIPIDS
LIPOPROTEINS
LIVER
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTOSIS
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
STEROID HORMONES
STEROIDS
UPTAKE
VERTEBRATES