Synthesis of (gamma-/sup 32/P)thiamine triphosphate
Journal Article
·
· Anal. Biochem.; (United States)
We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate /sup 32/P in the gamma position. The reaction is based on the condensation of (/sup 32/P)orthophosphoric acid and thiamine diphosphate in the presence of ethyl chloroformate. After purification by two ion-exchange purification steps, the thiamine derivative has a specific radioactivity of 10 Ci/mmol. The average final yield synthesis is about 10%.
- Research Organization:
- Universite de Liege (Belgium)
- OSTI ID:
- 7140937
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 169:2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AZINES
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL PREPARATION
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INORGANIC ACIDS
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORIC ACID
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PURIFICATION
PYRIMIDINES
RADIOISOTOPES
SEPARATION PROCESSES
SYNTHESIS
THIAMINE
THIAZOLES
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMINS
59 BASIC BIOLOGICAL SCIENCES
AMINES
AZINES
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL PREPARATION
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INORGANIC ACIDS
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORIC ACID
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PURIFICATION
PYRIMIDINES
RADIOISOTOPES
SEPARATION PROCESSES
SYNTHESIS
THIAMINE
THIAZOLES
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMINS