Role of acyl carrier protein isoforms in plant lipid metabolism
Although acyl carrier protein (ACP) is the best studied protein in plant fatty acid biosynthesis, the in vivo forms of ACPs and their steady state pools have not been examined previously in either seed or leaf. Information about the relative pool sizes of free ACP and its acyl-ACP intermediates is essential for understanding regulation of de novo fatty acid biosynthesis in plants. In this study we utilized antibodies directed against spinach ACP as a sensitive assay to analyze the acyl groups while they were still covalently attached to ACPs. 4 refs., 4 figs.
- Research Organization:
- Michigan State Univ., East Lansing, MI (USA)
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG02-87ER13729
- OSTI ID:
- 7138275
- Report Number(s):
- DOE/ER/13729-2; ON: DE90010959
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
PROTEINS
BIOASSAY
ACYLATION
BIOSYNTHESIS
CARBOXYLIC ACIDS
ELECTROPHORESIS
IN VIVO
LEAVES
LIPIDS
METABOLISM
PROGRESS REPORT
SEEDS
SEPARATION PROCESSES
SPINACH
CHEMICAL REACTIONS
DOCUMENT TYPES
FOOD
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SYNTHESIS
VEGETABLES
550200* - Biochemistry
PROTEINS
BIOASSAY
ACYLATION
BIOSYNTHESIS
CARBOXYLIC ACIDS
ELECTROPHORESIS
IN VIVO
LEAVES
LIPIDS
METABOLISM
PROGRESS REPORT
SEEDS
SEPARATION PROCESSES
SPINACH
CHEMICAL REACTIONS
DOCUMENT TYPES
FOOD
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SYNTHESIS
VEGETABLES
550200* - Biochemistry