Circadian disc shedding in Xenopus retina in vitro
Journal Article
·
· Invest. Ophthalmol. Visual Sci.; (United States)
OSTI ID:7135217
To further examine the endogenous rhythm of disc shedding and phagocytosis observed in several species, adult Xenopus were entrained to a 12 hr light/12 hr dark cycle and then placed in constant darkness. At various times during a 3-day period of constant darkness, eyes were explanted and placed into culture medium, then processed for light and electron microscopy. A clear rhythmicity of disc shedding was observed, with pronounced peaks at the times light onset occurred in the original entrainment cycle. Modification of the HCO/sub 3/- ion concentration in the medium was found to raise the amplitude of the peak of endogenous disc shedding. Explants maintained in culture medium containing deuterium oxide (a compound known to perturb circadian oscillators) were found to shed with a longer interval between peaks. The addition of the protein synthesis inhibitor, anisomycin, to this preparation suppressed the shedding rhythm. The action of anisomycin was investigated by autoradiographic examination of the pattern of /sup 3/H-leucine uptake and protein synthesis by the explant. The findings suggest the presence of a circadian oscillator for rhythmic disc shedding within the amphibian eye.
- Research Organization:
- Department of Biological Sciences, University of California, Santa Barbara, CA
- OSTI ID:
- 7135217
- Journal Information:
- Invest. Ophthalmol. Visual Sci.; (United States), Journal Name: Invest. Ophthalmol. Visual Sci.; (United States) Vol. 25:2; ISSN IOVSD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID CARBONATES
AMINO ACIDS
AUTORADIOGRAPHY
BIOCHEMISTRY
BODY
BODY AREAS
CARBOXYLIC ACIDS
CHEMISTRY
DAILY VARIATIONS
DEUTERIDES
DEUTERIUM COMPOUNDS
ELECTRON MICROSCOPY
EYES
FACE
HEAD
HYDROGEN COMPOUNDS
HYDROGEN DEUTERIDE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
MICROSCOPY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTOSIS
RETINA
SENSE ORGANS
TISSUE CULTURES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VARIATIONS
59 BASIC BIOLOGICAL SCIENCES
ACID CARBONATES
AMINO ACIDS
AUTORADIOGRAPHY
BIOCHEMISTRY
BODY
BODY AREAS
CARBOXYLIC ACIDS
CHEMISTRY
DAILY VARIATIONS
DEUTERIDES
DEUTERIUM COMPOUNDS
ELECTRON MICROSCOPY
EYES
FACE
HEAD
HYDROGEN COMPOUNDS
HYDROGEN DEUTERIDE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
MICROSCOPY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTOSIS
RETINA
SENSE ORGANS
TISSUE CULTURES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VARIATIONS