Identification of amino acid residues photolabeled with 2-azido(alpha-/sup 32/P)adenosine diphosphate in the beta subunit of beef heart mitochondrial F1-ATPase
When beef heart mitochondrial F1-ATPase is photoirradiated in the presence of 2-azido(alpha-/sup 32/P)adenosine diphosphate, the beta subunit of the enzyme is preferentially photolabeled (Dalbon, P., Boulay, F., and Vignais, P. V. (1985) FEBS Lett. 180, 212-218). The site of photolabeling of the beta subunit has been explored. After cyanogen bromide cleavage of the photolabeled beta subunit, only the peptide fragment extending from Gln-293 to Met-358 was found to be labeled. This peptide was isolated and digested by trypsin or Staphylococcus aureus V8 protease. Digestion by trypsin yielded four peptides, one of which spanned residues Ala-338-Arg-356 and contained all the bound radioactivity. When trypsin was replaced by V8 protease, a single peptide spanning residues Leu-342-Met-358 was labeled. Edman degradation of the two labeled peptides showed that radioactivity was localized on the following four amino acids: Leu-342, Ile-344, Tyr-345, and Pro-346.
- Research Organization:
- Centre d'Etudes Nucleaires, Grenoble, France
- OSTI ID:
- 7135157
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 15; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ADP
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
ATP-ASE
AZIDES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CATTLE
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ENZYMES
HYDROLASES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MAMMALS
MITOCHONDRIA
MOLECULAR STRUCTURE
NITROGEN COMPOUNDS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PEPTIDE HYDROLASES
PEPTIDES
PHOSPHOHYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RUMINANTS
SERINE PROTEINASES
TRYPSIN
VERTEBRATES