Model system for the study of RNA digestion in the intestine

Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7134756

Chen, H P; Gross, C J; Savaiano, D A

A model system was developed to study the kinetics and regulation of the intestinal digestion of RNA. RNA from Saccharomyces cerevisiae mutant strains, Guo 2.1 and Ade 12, were selectively radiolabeled in the adenine or guanine bases according to the method of Burridge and Woods. RNA was extracted from the yeast by incubation at 65/sup 0/C in Tris buffer (pH 7.2) and water-saturated phenol for 30 min and precipitated in ethanol at -70/sup 0/C overnight. The radiolabel in the resultant extract was exclusively in RNA with no labeled free nucleotides or bases detectable according to HV paper electrophoresis. Digestion of the RNA by commercial bovine ribonuclease resulted in the recovery of > 95% of the label in the acid-soluble fraction. 300 mg of the labeled RNA (0.7 ..mu..Ci) was injected into a ligated 15-cm segment of the proximal rat small intestine. Intestinal contents were sampled at 10, 20, and 30 min. Portions of the acid-soluble fraction and acid-insoluble precipitate were analyzed for radioactivity. Greater than 90% of the purified yeast RNA was digested by 30 min in the intestinal lumen. Furthermore, 80% of the radiolabel disappeared from the intestinal lumen. The kinetics and regulation of the digestion of RNA will be studied using this model system.

• Research Organization: Univ. of Minnesota, St. Paul
• OSTI ID: 7134756
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MODELS
BODY
DIGESTIVE SYSTEM
ELECTROPHORESIS
FUNGI
GASTROINTESTINAL TRACT
INTESTINES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
METABOLISM
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PLANTS
RADIOISOTOPES
RNA
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SMALL INTESTINE
TRACER TECHNIQUES
YEASTS