Endothelial cell cytotoxicity of cotton bracts tannin and aqueous cotton bracts extract
Using an in vitro cytotoxicity assay based on the release of /sup 51/Cr from cultured porcine thoracic aortic and pulmonary arterial endothelial cells, we have demonstrated that cotton bracts tannin is a potent endothelial cell cytotoxin. It produces dose-dependent lethal injury to both types of endothelial cells with the aortic cells, being somewhat more sensitive to tannin-mediated injury than the pulmonary arterial cells. Cytotoxic injury to the cells was biphasic. During the first 3 hr of exposure to tannin, no lethal injury was detected. However, during this period, profound changes in morphology were observed suggesting sublethal injury to the cells preceded the ultimate toxic damage. Comparison of the cytotoxicity dose curves for aqueous bracts extracts with those for tannin demonstrated that tannin was major cytotoxin present in bracts.
- Research Organization:
- Mayo Clinic and Foundation, Rochester, MN
- OSTI ID:
- 7134252
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 66
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
MORPHOLOGICAL CHANGES
SCANNING ELECTRON MICROSCOPY
TANNIC ACID
RADIOASSAY
TOXICITY
AORTA
CHROMIUM 51
COTTON
ENDOTHELIUM
LUNGS
SWINE
ANIMAL TISSUES
ANIMALS
AROMATICS
ARTERIES
BETA DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHROMIUM ISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
EVEN-ODD NUCLEI
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MICROSCOPY
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
RADIOISOTOPES
RESPIRATORY SYSTEM
TISSUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
560300 - Chemicals Metabolism & Toxicology