Differential hormonal regulation of L-glycerol-3-phosphate dehydrogenase in rat brain and skeletal muscle

Technical Report · Thu Jan 01 04:00:00 EST 1976

OSTI ID:7131095

McGinnis, J F; de Vellis, J

The level of L-glycerol-3-phosphate dehydrogenase (EC. 1.1.1.8) is regulated in the rat brain by glucocorticoids. Following hypophysectomy the concentration of brain glycerol-3-P dehydrogenase decreases to about 40 percent of the control. By immunotitration, we have demonstrated that this decrease in glycerol-3-P dehydrogenase activity is due to fewer enzyme molecules rather than less efficient ones. We also demonstrated that the enzyme remaining in the brain after hypophysectomy is identical to that found in the brains of control littermates as determined by gel permeation chromatography, pH optimum, heat lability, electrophoretic mobility and Ouchterlony double diffusion analysis. Since the concentration of glycerol-3-P dehydrogenase in skeletal muscle is not regulated by glucocorticoids, we also compared the brain enzyme to the muscle enzyme. By the above criteria, skeletal muscle glycerol phosphate dehydrogenase is identical to the brain enzyme. This suggests that the same structural gene codes for glycerol phosphate dehydrogenase in brain and muscle and that the difference in response to glucocorticoids is due to the presence of a specific regulatory mechanism in brain that is absent in muscle.

• Research Organization: California Univ., Los Angeles (USA). Lab. of Nuclear Medicine and Radiation Biology
• OSTI ID: 7131095
• Report Number(s): UCLA-12-1107
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CORTICOSTEROIDS
DEHYDROGENASES
ENZYMES
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
HYPOPHYSECTOMY
KETONES
MAMMALS
MEDICINE
METABOLISM
MUSCLES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PREGNANES
RATS
RODENTS
STEROID HORMONES
STEROIDS
SURGERY
VERTEBRATES