Stabilization of microorganisms for in situ degradation of toxic chemicals

Technical Report · Mon Jan 01 04:00:00 EST 1990

OSTI ID:7128794

Crawford, R L; Stormo, K

In our initial work, we have developed methods to microencapsulate cells within beads of 5--100,{mu}m diameter, and we have examined these entrapped cells for their abilities to mineralize specific chemicals in the presence of subsurface soils and waters obtained from the University of Idaho Groundwater Research Site (GRS). We have employed a pentachlorophenol (PCP)-degrading Flavobacterium and a toluene-degrading Pseudomonas. Cells were immobilized within one of three polymeric matrixes: alginate, agarose, or polyurethane.

• Research Organization: Idaho Univ., Moscow, ID (United States)
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG07-89ER60847
• OSTI ID: 7128794
• Report Number(s): DOE/ER/60847-1; ON: DE92040791
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540220* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
540250 -- Environment
Terrestrial-- Site Resource & Use Studies-- (1990-)
AQUIFERS
AROMATICS
BACTERIA
BIODEGRADATION
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CATALYST SUPPORTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DOCUMENT TYPES
HALF-LIFE
HAZARDOUS MATERIALS
HYDROXY COMPOUNDS
IMMOBILIZED CELLS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEVELS
MATERIALS
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PHENOLS
PROGRESS REPORT
RADIOISOTOPES
STABILITY
TOXIC MATERIALS
TRACER TECHNIQUES
UNDERGROUND
YEARS LIVING RADIOISOTOPES