Mechanism of action of ruthenium red compounds on Ca/sup 2 +/ ionophore from sarcoplasmic reticulum (Ca/sup 2 +/ + Mg/sup 2 +/)-adenosine triphosphatase and lipid bilayer
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:7126203
Sarcoplasmic reticulum (Ca/sup 2 +/ + Mg/sup 2 +/)-ATPase was previously shown to have Ca/sup 2 +/-dependent and -selective ionophoric activity when tested in oxidized cholesterol lipid bilayer membranes (Shamoo, A. E., and MacLennan, D. H. (1974) Proc. Natl. Acad. Sci. U. S. A. 71, 3522). Ruthenium red, a known inhibitor of (Ca/sup 2 +/ + Mg/sup 2 +/)-ATPase, is found to inhibit the Ca/sup 2 +/-ionophoric activity associated with (Ca/sup 2 +/ + Mg/sup 2 +/)-ATPase. Furthermore, ruthenium red alone acts as an anion-selective ionophore in lipid bilayers with the following selectivity sequence for anions: l/sup -/ > Cl/sup -/, Br/sup -/ > F/sup -/ > NO/sub 3//sup -/. The P/sub Cl/sup -///P/sub Na/sup +// ratio was approx.4/1. The presence of ruthenium red in excess of Ca/sup 2 +/ ionophore in lipid bilayer experiments converts the cation selectivity of the bilayer due to Ca/sup 2 +/ ionophore into anion selectivity.
- Research Organization:
- Univ. of Rochester, NY
- OSTI ID:
- 7126203
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 250:20; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Separate effects of mercurial compounds on the ionophoric and hydrolytic functions of the (Ca/sup + +/ + Mg/sup + +/)-ATPase of sarcoplasmic reticulum. [Skeletal muscle of rabbits]
Ionophorous properties of the 20,000-dalton fragment of (Ca/sup 2 +/ + Mg/sup 2 +/)-ATPase in phosphatidylcholine: cholesterol membranes
Ca/sup + +/-dependent and -selective ionophore as part of the Ca/sup + +/ + Mg/sup + +/-dependent adenosinetriphosphatase of sarcoplasmic reticulum
Journal Article
·
Tue Dec 31 23:00:00 EST 1974
· J. Membr. Biol.; (United States)
·
OSTI ID:7212948
Ionophorous properties of the 20,000-dalton fragment of (Ca/sup 2 +/ + Mg/sup 2 +/)-ATPase in phosphatidylcholine: cholesterol membranes
Journal Article
·
Sat Dec 31 23:00:00 EST 1977
· J. Membr. Biol.; (United States)
·
OSTI ID:5774909
Ca/sup + +/-dependent and -selective ionophore as part of the Ca/sup + +/ + Mg/sup + +/-dependent adenosinetriphosphatase of sarcoplasmic reticulum
Journal Article
·
Sun Sep 01 00:00:00 EDT 1974
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:7193135
Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ATOMIC IONS
ATP
ATP-ASE
BIOCHEMICAL REACTION KINETICS
CALCIUM IONS
CHARGED PARTICLES
CHOLESTEROL
ENZYMES
ESTERASES
HYDROLASES
HYDROXY COMPOUNDS
INHIBITION
IONS
KINETICS
LIPIDS
MAGNESIUM IONS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHATASES
REACTION KINETICS
RUTHENIUM COMPOUNDS
STEROIDS
STEROLS
TRANSITION ELEMENT COMPOUNDS
59 BASIC BIOLOGICAL SCIENCES
ATOMIC IONS
ATP
ATP-ASE
BIOCHEMICAL REACTION KINETICS
CALCIUM IONS
CHARGED PARTICLES
CHOLESTEROL
ENZYMES
ESTERASES
HYDROLASES
HYDROXY COMPOUNDS
INHIBITION
IONS
KINETICS
LIPIDS
MAGNESIUM IONS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHATASES
REACTION KINETICS
RUTHENIUM COMPOUNDS
STEROIDS
STEROLS
TRANSITION ELEMENT COMPOUNDS