Metabolism of labeled parathyroid hormone. III. Studies in rats

Journal Article · Wed Jan 01 04:00:00 EST 1975 · Calcif. Tissue Res.; (United States)

DOI:https://doi.org/10.1007/BF02546244· OSTI ID:7126186

Neuman, W F; Neuman, M W; Sammon, P J; Simon, W; Lane, K

A series of experiments indicated that /sup 125/I-parathyroid hormone administered to the rat i.v. was deposited very rapidly in three organ systems only, bone, kidney and the liver. As indicated by increasing solubility in trichloracetic acid, the hormone seemed to be rapidly metabolized at the sites of deposition. The experiments were in part repeated employing quantitative extraction and gel filtration procedures. It was found that intact hormone predominated (82 percent) in the circulation but was cleared very rapidly: 91, 97, and 99 percent having left by 10, 30 and 60 minutes respectively after injection. The intact hormone was found at 10 minutes to be principally in kidney, bone, and liver. Although there was evidence of fragmentation preferentially to polypeptides of molecular weights of 6000, 45000 and 2500 these cannot be claimed to represent real molecular entities. By one hour, only a few percent of the injected hormone remained intact, the bulk having been degraded to fragments of small molecular weight.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 7126186
• Journal Information: Calcif. Tissue Res.; (United States), Journal Name: Calcif. Tissue Res.; (United States) Vol. 18; ISSN CATRB
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PARATHORMONE
PEPTIDE HORMONES
RADIOISOTOPES
RATS
RODENTS
SKELETON
VERTEBRATES