Further characterization of tuftsin receptors

Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7119551

Bump, N J; Lee, J; Najjar, V A

Tuftsin receptor was purified from rabbit peritoneal granulocytes by affinity chromatography. The pentapeptide analog, Thr-Lys-Pro-Pro-Arg was covalently linked to a solid support column. Rabbit granulocyte membrane was prepared, dissolved in 8 mM CHAPS and run through the column, eluted with 20 eta M free pentapeptide and subjected to dialysis concentration. When this was run on SDS-PAGE, two bands were obtained at a migration equivalent to Mr 60 and 62 K. These were electroblotted on nitrocellulose paper which showed two corresponding (/sup 3/H)-tuftsin binding bands. After reduction, and boiling, SDS-PAGE runs showed two bands Mr 85 and 70 K. When the purified receptor was reduced, alkylated and treated with endo-..beta..-N-acetylglucosaminidase H, only one band was obtained at Mr of about 90 K.

• Research Organization: Tufts Univ. School of Medicine, Boston, MA
• OSTI ID: 7119551
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKYLATION
ANIMALS
BIOCHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
CHROMATOGRAPHY
COVALENCE
ELECTROPHORESIS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
PURIFICATION
RABBITS
REACTION KINETICS
RECEPTORS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES