Techniques for intrinsically labeling wheat with /sup 65/Zn
Several techniques of intrinsically labeling wheat with /sup 65/Zn were compared: stem injection of /sup 65/Zn, stem injection of /sup 65/Zn + ZnSO/sub 4/, foliar application of /sup 65/Zn, and the addition of /sup 65/Zn to a hydroponic solution. Incorporation levels of /sup 65/Zn into the grain were 62.6% stem injection, 45.2% stem-injected /sup 65/Zn + ZnSO/sub 4/, 57.5% foliar application, and 2.3% hydroponic solution. Four protein fractions were extracted from fat-free whole wheat flour. Distribution of /sup 65/Zn into the protein fractions for all treatments, was 8.5-20.3% in albumins and globulins, 47.4-60.3% in glutenins, 1-2.6% in gliadins, and 9.8-28.3% in the remaining proteins. Separation of the fractions by gel chromatography showed that protein and Zn distributions were similar among the treatments and when compared to the controls. Zinc-65 distribution was similar to the natural Zn distribution. These data illustrate that stem-injected /sup 65/Zn is incorporated in the same manner and ratios as Zn naturally utilized by wheat.
- Research Organization:
- Dept. of Agriculture, Grand Forks, ND
- OSTI ID:
- 7118355
- Journal Information:
- J. Agric. Food Chem.; (United States), Journal Name: J. Agric. Food Chem.; (United States) Vol. 33:4; ISSN JAFCA
- Country of Publication:
- United States
- Language:
- English
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60 APPLIED LIFE SCIENCES
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
CEREALS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GRASS
INTERMEDIATE MASS NUCLEI
ISOTOPES
LABELLING
NUCLEI
ORGANIC COMPOUNDS
PLANTS
PROTEINS
RADIOISOTOPES
TISSUE DISTRIBUTION
WHEAT
ZINC 65
ZINC ISOTOPES