A photoactivatable probe for the Na+/H+ exchanger cross-links a 66-kDa renal brush border membrane protein
Journal Article
·
· Journal of Biological Chemistry; (USA)
OSTI ID:7118073
- Washington Univ. School of Medicine, St. Louis, MO (USA)
Earlier studies on LLC-PK1 cells have demonstrated two pharmacologically distinct Na+/H+ exchangers in renal epithelia. In addition, the cDNA clone for the human Na+/H+ antiporter which is growth factor activatable has been isolated and expressed. We report here the synthesis of an amiloride analogue that can be photoactivated and labeled with 125I. This analogue covalently cross-links a 66-kDa protein of bovine renal brush border membranes. A rabbit polyclonal antibody that was directed against a 20-amino acid peptide of the cytoplasmic domain of its human Na+/H+ antiporter also gives a positive Western against 66-kDa protein of bovine brush border membranes. Thus, the photoactive probe may be helpful in the isolation and purification of the brush border Na+/H+ exchanger.
- OSTI ID:
- 7118073
- Journal Information:
- Journal of Biological Chemistry; (USA), Journal Name: Journal of Biological Chemistry; (USA) Vol. 265:10; ISSN JBCHA; ISSN 0021-9258
- Country of Publication:
- United States
- Language:
- English
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Tue Apr 04 00:00:00 EDT 1989
· Biochemistry; (USA)
·
OSTI ID:5405608
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· Biochem. Biophys. Res. Commun.; (United States)
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· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6181749
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BODY
CATTLE
CHEMICAL PREPARATION
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DIURETICS
DOGS
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
MAMMALS
MASS SPECTRA
MEMBRANE PROTEINS
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEIN STRUCTURE
PROTEINS
RABBITS
RADIOISOTOPES
REAGENTS
RUMINANTS
SPECTRA
SYNTHESIS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BODY
CATTLE
CHEMICAL PREPARATION
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DIURETICS
DOGS
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
MAMMALS
MASS SPECTRA
MEMBRANE PROTEINS
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEIN STRUCTURE
PROTEINS
RABBITS
RADIOISOTOPES
REAGENTS
RUMINANTS
SPECTRA
SYNTHESIS
VERTEBRATES