Effects of fast neutrons on spermatogenesis of the fish, Oryzias latipes. [X radiation]

Journal Article · Sun May 01 04:00:00 EDT 1977 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574592· OSTI ID:7110122

Hyodo-Taguchi, Y; Maruyama, T

The weight loss of the testes, the behavior of the primary spermatogonia Ia and Ib, and the inhibition of DNA synthesis in the testes of the fish, Oryzias latipes, which had been kept at low temperature, irradiated with 2 MeV of fast neutrons (100, 200, 400, and 600 rad), and transferred to 25/sup 0/C, were investigated and compared with the effects of 200 kVp of x rays. A considerable weight loss of the testes was demonstrated at 15 days after irradiation with neutrons. The 50% effective dose (ED/sub 50/) for weight loss of testes was less than 100 rad after fast neutrons and about 500 rad after 200 kVp of x rays. Although the primary spermatogonia Ia were not significantly reduced in number after neutron irradiation, the primary spermatogonia Ib were markedly damaged. At 3 days after irradiation, the pyknoses of the primary spermatogonia Ib and surviving fraction of the cells depended on the exposure dose. Fast neutrons had ED/sub 50/ values of 330 rad at 3 days and 130 rad at 5 days after irradiation in the surviving fraction of the primary spermatogonia Ib and after x irradiation; ED/sub 50/ values were 810 and 490 rad at 3 and 5 days, respectively. In all dose groups, the lowest number of the cells was observed at 15 days. Thereafter the number of the spermatogonia Ib increased, returning to the control level and 55% of the control in the fish irradiated with 100 and 200 rad of neutrons at 30 days.

• Research Organization: National Inst. of Radiological Sciences, Chiba, Japan
• OSTI ID: 7110122
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 70:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
DNA
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
FAST NEUTRONS
FERMIONS
FISHES
GAMETES
GAMETOGENESIS
GERM CELLS
GONADS
HADRONS
IONIZING RADIATIONS
MALE GENITALS
NEUTRONS
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RBE
SPERMATOGENESIS
SPERMATOGONIA
SYNTHESIS
TESTES
VERTEBRATES
WEIGHT
X RADIATION