Turnover of extracellular DNA in eutrophic and oligotrophic freshwater environments of southwest Florida

Technical Report · Sun Jan 01 00:00:00 EST 1989

OSTI ID:7109805

Paul, J H; Jeffrey, W H; David, A W; DeFlaun, M F; Cazares, L H

Turnover of extracellular DNA was investigated in oligotrophic springs of the Crystal River and the eutrophic Medard Reservoir of southwest Florida. The Medard Reservoir possessed large populations of bacterioplankton and phytoplankton (6.8 x 10 sup 9 cells per liter and 28.6 micrograms of chlorophyll a per liter, respectively), while the Crystal River springs only contained a fraction of the microbial biomass found in the Medard Reservoir. The results indicate that regardless of trophic status or microbial standing stock, extracellular DNA turns over rapidly in subtropical planktonic freshwater environments. Therefore, recombinant DNA sequences from released genetically engineered microorganisms might not be expected to survive for long periods of time in freshwater planktonic environments.

• Research Organization: University of South Florida, St. Petersburg, FL (USA). Dept. of Marine Science
• OSTI ID: 7109805
• Report Number(s): PB-90-140823/XAB
• Resource Relation: Other Information: Pub. in Applied and Environmental Microbiology, Vol. 55, No. 7, 1823-1828(Jul 1989)
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
DNA
BIOSYNTHESIS
MICROORGANISMS
GENETIC ENGINEERING
BIOMASS
CHLOROPHYLL
FLORIDA
FRESH WATER
PLANKTON
RECOMBINANT DNA
AQUATIC ORGANISMS
CARBOXYLIC ACIDS
ENERGY SOURCES
FEDERAL REGION IV
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
NORTH AMERICA
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
PROTEINS
RENEWABLE ENERGY SOURCES
SYNTHESIS
USA
WATER
140505* - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)