Partial purification of the ATP-driven calcium pump of Streptococcus sanguis

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7108609

Lynn, A R; Rosen, B P

ATP-dependent transport of calcium has been observed in several species of streptococci as uptake of /sup 45/Ca/sup 2 +/ into everted membrane vesicles. Membranes from Streptococcus sanguis and Streptococcus faecalis were solubilized with octyl-..beta..-D-glucoside or Triton X-100, and the extracts reconstituted into proteoliposomes containing Escherichia coli or soybean phospholipid. Calcium transport in reconstituted proteoliposomes was insensitive to the ionophores nigericin and valinomycin and was unaffected by the F/sub 0/F/sub 1/ inhibitor N,N'-dicyclohexylcarbodiimide. Uptake was inhibited by ortho-vanadate with a K/sub i/ in the micromolar range. These results demonstrate that the reconstituted transport activities are not the result of ATP-driven proton pumping via the F/sub 0/F/sub 1/ coupled to a calcium/proton antiporter and suggest that existence of a calcium translocating ATPase. Partial purification of the transport activity from Streptococcus sanguis has been achieved using density gradient centrifugation and FPLC.

• Research Organization: Univ. of Maryland School of Medicine, Baltimore
• OSTI ID: 7108609
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:7
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ATP
ATP-ASE
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
HYDROLASES
IMIDES
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIPOSOMES
MEMBRANE TRANSPORT
MEMBRANES
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
OXYGEN COMPOUNDS
PHOSPHOHYDROLASES
PURIFICATION
RADIOISOTOPES
STREPTOCOCCUS
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
VALINOMYCIN
VANADATES
VANADIUM COMPOUNDS