Sucrose translocation in Pseudomonas saccharophila

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7108603

Liu, D F; Hitz, W D; Giaquinta, R T; Viitanen, P V

Unlike many microorganisms, P. saccharophila is capable of growth on sucrose but not on glucose or fructose without prior mutation. For the wild-type sucrose uptake into cells requires induction by substrate and this is accompanied by the appearance of a membrane-bound protein with an apparent MW of approx. 84kDa. A constitutive, overproducer of this protein has been isolated and as expected this mutant transports sucrose in an amplified manner. Scatchard analysis of /sup 3/H-sucrose binding to cytoplasmic membranes reveals an apparent K/sub D/ of about 1.0..mu..M for both mutant and wild-type. In contrast mutant membranes contain 5-6 times more binding sites than the wild-type membranes. 6'-deoxy-6'-(2-hydroxy-4-azido)benzamido-sucrose (6'-HABS), a sucrose analogue, has been used to identify the protein responsible for sucrose binding. When cytoplasmic membranes are irradiated in the presence of /sup 125/I-6'-HABS, a significant portion of the radioactivity is found covalently associated with the 84kDa band in SDS-PAGE. Importantly, labeling of this band is largely prevented when unlabeled sucrose is present during photolysis. The authors believe that the 84kDa protein may be a sucrose permease and efforts are underway to reconstitute it in a functional form.

• Research Organization: E.I. Du Pont de Nemours, Wilmington, DE
• OSTI ID: 7108603
• Report Number(s): CONF-8606151-; TRN: 86-039066
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:7; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
LABELLING
SACCHAROSE
BIOCHEMICAL REACTION KINETICS
MEMBRANE TRANSPORT
CELL MEMBRANES
COVALENCE
CYTOPLASM
IODINE 125
MUTANTS
PSEUDOMONAS
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BACTERIA
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISACCHARIDES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MEMBRANE PROTEINS
MEMBRANES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques