Effect of adenosine and adenosine analogs on ( sup 14 C)aminopyrine accumulation by rabbit parietal cells

Journal Article · Fri Dec 01 04:00:00 EST 1989 · Digestive Diseases and Sciences; (USA)

DOI:https://doi.org/10.1007/BF01536706· OSTI ID:7105816

Ota, S; Hiraishi, H; Terano, A; Mutoh, H; Kurachi, Y; Shimada, T; Ivey, K J; Sugimoto, T ^[1]

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1. Univ. of Tokyo (Japan)

Adenosine receptors that modulate adenylate cyclase activity have been identified recently in a number of tissues. Adenosine A2 receptor is stimulatory to adenylate cyclase, whereas adenosine A1 receptor is inhibitory to adenylate cyclase. We investigated the effect of adenosine and its analogs on (14C)aminopyrine accumulation by rabbit parietal cells. Rabbit gastric mucosal cells were isolated by enzyme digestion. Parietal cells were enriched by nonlinear percoll gradients. (14C)Aminopyrine accumulation was used as an indicator of acid secretion. The effect of 2-chloroadenosine on histamine-stimulated (14C)aminopyrine accumulation was studied. The effects of N-ethylcarboxamideadenosine, 2-chloroadenosine, stable analogs of adenosine, and adenosine on (14C)aminopyrine accumulation were assessed. Cyclic AMP content of parietal cells was determined by radioimmunoassay. Histamine and carbachol, known secretagogues, stimulated (14C)aminopyrine accumulation. 2-Chloroadenosine did not suppress histamine-stimulated (14C)aminopyrine accumulation. 2-Chloroadenosine, N-ethylcarboxamideadenosine, and adenosine dose dependently increased (14C)aminopyrine accumulation. The order of potency was N-ethylcarboxamideadenosine greater than 2-chloroadenosine greater than adenosine. 8-Phenyltheophylline and theophylline, adenosine-receptor antagonists, or cimetidine did not have significant effects on the increase of AP uptake induced by 2-chloroadenosine. Coadministration of dipyridamole, and adenosine uptake inhibitor, augmented the effect of adenosine on (14C)aminopyrine accumulation. 2-Chloroadenosine, N-ethylcarboxamideadenosine, and adenosine each induced a significant increase in cellular cyclic AMP. We conclude that there may be adenosine A2 receptors on rabbit parietal cells which modulate gastric acid secretion.

• OSTI ID: 7105816
• Journal Information: Digestive Diseases and Sciences; (USA), Journal Name: Digestive Diseases and Sciences; (USA) Vol. 34:12; ISSN 0163-2116; ISSN DDSCD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
AMP
ANIMAL CELLS
ANIMALS
ANTIPYRETICS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CYCLASES
DIGESTIVE SYSTEM
DRUGS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
GASTRIC ACID
GASTROINTESTINAL TRACT
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LYASES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MUCOUS MEMBRANES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
PROTEINS
RABBITS
REACTION KINETICS
RECEPTORS
RIBOSIDES
TRACER TECHNIQUES
VERTEBRATES