Effect of adenosine and adenosine analogs on ( sup 14 C)aminopyrine accumulation by rabbit parietal cells

Journal Article · Fri Dec 01 00:00:00 EST 1989 · Digestive Diseases and Sciences; (USA)

DOI:https://doi.org/10.1007/BF01536706· OSTI ID:7105816

Ota, S; Hiraishi, H; Terano, A; Mutoh, H; Kurachi, Y; Shimada, T; Ivey, K J; Sugimoto, T ^[1]

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1. Univ. of Tokyo (Japan)

Adenosine receptors that modulate adenylate cyclase activity have been identified recently in a number of tissues. Adenosine A2 receptor is stimulatory to adenylate cyclase, whereas adenosine A1 receptor is inhibitory to adenylate cyclase. We investigated the effect of adenosine and its analogs on (14C)aminopyrine accumulation by rabbit parietal cells. Rabbit gastric mucosal cells were isolated by enzyme digestion. Parietal cells were enriched by nonlinear percoll gradients. (14C)Aminopyrine accumulation was used as an indicator of acid secretion. The effect of 2-chloroadenosine on histamine-stimulated (14C)aminopyrine accumulation was studied. The effects of N-ethylcarboxamideadenosine, 2-chloroadenosine, stable analogs of adenosine, and adenosine on (14C)aminopyrine accumulation were assessed. Cyclic AMP content of parietal cells was determined by radioimmunoassay. Histamine and carbachol, known secretagogues, stimulated (14C)aminopyrine accumulation. 2-Chloroadenosine did not suppress histamine-stimulated (14C)aminopyrine accumulation. 2-Chloroadenosine, N-ethylcarboxamideadenosine, and adenosine dose dependently increased (14C)aminopyrine accumulation. The order of potency was N-ethylcarboxamideadenosine greater than 2-chloroadenosine greater than adenosine. 8-Phenyltheophylline and theophylline, adenosine-receptor antagonists, or cimetidine did not have significant effects on the increase of AP uptake induced by 2-chloroadenosine. Coadministration of dipyridamole, and adenosine uptake inhibitor, augmented the effect of adenosine on (14C)aminopyrine accumulation. 2-Chloroadenosine, N-ethylcarboxamideadenosine, and adenosine each induced a significant increase in cellular cyclic AMP. We conclude that there may be adenosine A2 receptors on rabbit parietal cells which modulate gastric acid secretion.

• OSTI ID: 7105816
• Journal Information: Digestive Diseases and Sciences; (USA), Vol. 34:12; ISSN 0163-2116
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
BIOCHEMICAL REACTION KINETICS
ANTIPYRETICS
BIOLOGICAL ACCUMULATION
CYCLASES
ENZYME ACTIVITY
AMP
ANIMAL CELLS
CARBON 14 COMPOUNDS
ENZYME INHIBITORS
GASTRIC ACID
GASTROINTESTINAL TRACT
MUCOUS MEMBRANES
RABBITS
RECEPTORS
TRACER TECHNIQUES
ANIMALS
BIOLOGICAL MATERIALS
BODY FLUIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIGESTIVE SYSTEM
DRUGS
ENZYMES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LYASES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
RIBOSIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques