Investigation of a thrombin-complexing protein associated with platelets
Thesis/Dissertation
·
OSTI ID:7105715
A fraction of the {sup 125}I-thrombin that binds to human platelets is taken into a sodium dodecyl sulfate-resistant 77k Da complex with a platelet factor. This platelet factor is in several respects similar to protease nexin I (PNI), a fibroblasts thrombin inhibitor. The complexes are of the right size, bind to agarose that has been derivatized with either anti-PNI antibody or heparin, do not form when the thrombin active site has been blocked with diisopropylphosphofluoridate, and do not appear on platelets when heparin is present. The interaction with the platelet surface may modulate the conformation and function of this platelet form of protease nexin I (PNI{sub p}) because: (i) an antibody against protease nexi I inhibited released PNI{sub p}, but not platelet-bound PNI{sub p} from complexing {sup 125}I-thrombin, and (ii) whereas PNI{sub p} extracted from platelets bound both thrombin and urokinase, platelet-bound PNI{sub p} bound only thrombin. In experiments employing several different platelet isolation methods, PNI{sub p} accounted for a large fraction of the rapid high affinity binding of {sup 125}I-thrombin to platelets. However, platelets isolated and maintained in the presence of metabolic inhibitors failed to take added thrombin into {sup 125}I-thrombine-PNI{sub p} complexes.
- Research Organization:
- Kansas Univ., Lawrence, KS (USA)
- OSTI ID:
- 7105715
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
COAGULANTS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME INHIBITORS
ENZYMES
FIBRINOLYTIC AGENTS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SERINE PROTEINASES
THROMBIN
TRACER TECHNIQUES
UROKINASE
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
COAGULANTS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME INHIBITORS
ENZYMES
FIBRINOLYTIC AGENTS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SERINE PROTEINASES
THROMBIN
TRACER TECHNIQUES
UROKINASE
VERTEBRATES