Blood cell labeling with sup 99m Tc: Progress and perspectives

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Seminars in Nuclear Medicine; (USA)

DOI:https://doi.org/10.1016/S0001-2998(05)80175-8· OSTI ID:7105464

Srivastava, S C; Straub, R F ^[1]

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1. Brookhaven National Lab., Upton, NY (USA)

Blood cell labeling with {sup 99m}Tc has progressed through various developmental phases. In the case of red cell labeling, the science seems to have matured sufficiently, although minor refinements in the procedures will no doubt continue to be made. During the last 3 to 5 years, there has been a resurgence of interest in labeling leukocytes and platelets with {sup 99m}Tc. As a result of these efforts, the techniques for these cell types appear to be developing slowly, having finally come out of their infancy. Progress in these directions over the last 3 1/2 years is summarized and discussed in this article. Emerging techniques that offer the promise of combining simplicity and convenience with reliable and reproducible data are highlighted. Mechanisms involved in the various labeling approaches, if studied and understood, are included. Recent efforts on cell labeling with {sup 99m}Tc using the monoclonal antibody approach are summarized. Although results in this area are quite preliminary, the monoclonal antibody approach holds the greatest promise for labeling leukocytes and platelets in vivo, and thus overcoming the biggest drawback of current techniques, ie, cell separation and handling before labeling. 78 references.

• OSTI ID: 7105464
• Journal Information: Seminars in Nuclear Medicine; (USA), Journal Name: Seminars in Nuclear Medicine; (USA) Vol. 20:1; ISSN SMNMA; ISSN 0001-2998
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
DOCUMENT TYPES
ERYTHROCYTES
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LEUKOCYTES
MATERIALS
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
REVIEWS
TECHNETIUM 99
TECHNETIUM ISOTOPES
TRACER TECHNIQUES
YEARS LIVING RADIOISOTOPES