Ion microprobe analysis of bone surface elements: Effects of 1,25(OH)2D3

Journal Article · Fri Dec 01 04:00:00 EST 1989 · American Journal of Physiology; (USA)

OSTI ID:7105384

Bushinsky, D A; Chabala, J M; Levi-Setti, R ^[1]

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1. Univ. of Chicago, IL (USA)

When neonatal mouse calvariae are incubated with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) there is net calcium efflux from the bone into the medium. The effect of this enhanced cell-mediated Ca efflux on the relative concentrations of mineral 23Na, 39K, and 40Ca has not previously been studied. We used an imaging scanning ion microprobe, utilizing secondary ion mass spectrometry, to compare the relative ion concentrations of Na, K, and Ca on the surface, subsurface, and cross-section of cultured bone incubated in the presence of 1,25(OH)2D3 with the ion concentrations in similar regions of bone incubated in unaltered control medium. Changes in mineral ion concentration were correlated with net fluxes of Na, K, and Ca relative to bone. Calvariae incubated in control medium (24 h at pH approximately 7.40) have abundant surface Na and K relative to Ca (Na/Ca, 85 and K/Ca, 68), whereas the subsurface has less Na/Ca (21) and K/Ca (23), and on cross section the ratios of both Na/Ca (2.0) and K/Ca (1.9) decrease further. After incubation with 10(-8) M 1,25(OH)2D3, there is a significant increase in bone surface Na/Ca (154) and K/Ca (141) without a change in these ratios on the subsurface and a small fall in both ratios on cross section. The linear relationship between Na/Ca and K/Ca across the three regions of bone observed in control calvariae did not change with 1,25(OH)2D3 treatment. As determined by flux measurements there is a net efflux of Ca but not Na or K from bone.

• OSTI ID: 7105384
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 257; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550602* -- Medicine-- External Radiation in Diagnostics-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL ISOTOPES
ALKALI METALS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
BODY
CALCIUM
CALCIUM 40
CALCIUM ISOTOPES
CELL CULTURES
CHEMICAL ANALYSIS
ELECTRON MICROPROBE ANALYSIS
ELEMENTS
EVEN-EVEN NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MASS SPECTRA
MEMBRANE TRANSPORT
METALS
MICE
MICROANALYSIS
NEONATES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
POTASSIUM
POTASSIUM 39
POTASSIUM ISOTOPES
RODENTS
SKELETON
SODIUM
SODIUM 23
SODIUM ISOTOPES
SPECTRA
STABLE ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
VITAMIN D
VITAMINS