Effects of cell entrapment in Ca-alginate on the metabolism of yeast Saccharomyces cerevisiae
Saccharomyces cerevisiae cells grown in suspension have been immobilized in calcium-alginate beads. Fermentation rates and intracellular composition have been determined under nongrowing conditions in these Ca-alginate entrapped cells and for identical cells in suspension. Glucose uptake and ethanol and glycerol production are approximately two times faster in immobilized cells than in suspended cells. Intermediate metabolite levels such as fructose-1,6-diphosphate, glucose-6-phosphate and 3-phosphoglycerate have been determined by phosphorus-31 nuclear magnetic resonance (NMR) spectroscopy under glucose fermenting conditions. Carbon-13 NMR shows an increase in polysaccharide production in immobilized cells. S. cerevisiae cells grown within a Ca-alginate matrix have a specific growth rate 40% lower than the growth rate of similar cells cultivated in suspension. Alginate-grown cells have been used to compare glucose fermentation under nongrowing conditions in suspended and Ca-entrapped cells. Fermentation rate is higher in immobilized cells than in suspended cells. The observed differences in intracellular components between suspended and immobilized cells are qualitatively similar to the differences observed for cells grown in suspension. Ethanol production rate is 2.7 times faster in immobilized alginate-grown cells than in suspended suspension-grown cells.
- Research Organization:
- California Inst. of Tech., Pasadena, CA (USA)
- OSTI ID:
- 7100900
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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09 BIOMASS FUELS
ETHANOL
BIOSYNTHESIS
GLUCOSE
METABOLISM
GLYCEROL
SACCHAROMYCES CEREVISIAE
GROWTH
CARBON 13
IMMOBILIZED CELLS
METABOLITES
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
TRACER TECHNIQUES
ALCOHOLS
ALDEHYDES
CARBOHYDRATES
CARBON ISOTOPES
EUMYCOTA
EVEN-ODD NUCLEI
FUNGI
HEXOSES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
MONOSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
PLANTS
RESONANCE
SACCHARIDES
SACCHAROMYCES
SPECTRA
STABLE ISOTOPES
SYNTHESIS
YEASTS
550201* - Biochemistry- Tracer Techniques
550501 - Metabolism- Tracer Techniques
090900 - Biomass Fuels- Processing- (1990-)