Effect of thiol compounds and flavins on mercury and organomercurial degrading enzymes in mercury resistant aquatic bacteria

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Bulletin of Environmental Contamination and Toxicology; (USA)

DOI:https://doi.org/10.1007/BF01700139· OSTI ID:7098798

Pahan, K; Ray, S; Gachhui, R; Chaudhuri, J; Mandal, A ^[1]

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1. Univ. College of Science, Calcutta (India)

Plasmid-determined mercuric and organomercurial resistance in microorganisms has been studied by several workers. Mercury reductase, catalyzing the reduction of mercury depends on sulfhydryl compounds. Organomercurial lyase that catalyzes the splitting of C-Hg linkages also needs thiol compounds for its activity. Until recently, no study has been reported on thiol specificity of these enzymes from various sources. In the present study, the authors report on enzymatic volatilization of HgCl{sub 2} by fourteen Hg-resistant bacterial strains. They have also studied thiol specificity of Hg-reductases and organomercurial lyases isolated from the above bacterial species. Hg-reductase is known to have FAD-moiety which stimulates enzyme activity whereas FMN and riboflavin are ineffective in this regard. The effect of flavins, namely FAD, FMN and riboflavin, on Hg-reductase and organomercurial lyase activity is also reported here.

• OSTI ID: 7098798
• Journal Information: Bulletin of Environmental Contamination and Toxicology; (USA), Vol. 44:2, Issue 2; ISSN 0007-4861
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
FLAVINES
BIOLOGICAL EFFECTS
LYASES
ENZYME INDUCTION
MERCURY COMPOUNDS
BIODEGRADATION
OXIDOREDUCTASES
THIOLS
BACILLUS
CYSTEINE
ENZYME ACTIVITY
ESCHERICHIA COLI
GLUTATHIONE
KLEBSIELLA
MERCURY CHLORIDES
MICROCOCCUS
ORGANIC MERCURY COMPOUNDS
PROTEUS
RIBOFLAVIN
SALMONELLA
SHIGELLA
STREPTOCOCCUS
ACRIDINES
AMINES
AMINO ACIDS
AROMATICS
AZAARENES
AZINES
BACTERIA
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
DECOMPOSITION
DRUGS
ENZYMES
GENE REGULATION
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
MERCURY HALIDES
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
PYRIDINES
RADIOPROTECTIVE SUBSTANCES
VITAMIN B GROUP
VITAMINS
560300* - Chemicals Metabolism & Toxicology