Effect of thiol compounds and flavins on mercury and organomercurial degrading enzymes in mercury resistant aquatic bacteria

Journal Article · Wed Jan 31 23:00:00 EST 1990 · Bulletin of Environmental Contamination and Toxicology; (USA)

DOI:https://doi.org/10.1007/BF01700139· OSTI ID:7098798

Pahan, K; Ray, S; Gachhui, R; Chaudhuri, J; Mandal, A ^[1]

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1. Univ. College of Science, Calcutta (India)

Plasmid-determined mercuric and organomercurial resistance in microorganisms has been studied by several workers. Mercury reductase, catalyzing the reduction of mercury depends on sulfhydryl compounds. Organomercurial lyase that catalyzes the splitting of C-Hg linkages also needs thiol compounds for its activity. Until recently, no study has been reported on thiol specificity of these enzymes from various sources. In the present study, the authors report on enzymatic volatilization of HgCl{sub 2} by fourteen Hg-resistant bacterial strains. They have also studied thiol specificity of Hg-reductases and organomercurial lyases isolated from the above bacterial species. Hg-reductase is known to have FAD-moiety which stimulates enzyme activity whereas FMN and riboflavin are ineffective in this regard. The effect of flavins, namely FAD, FMN and riboflavin, on Hg-reductase and organomercurial lyase activity is also reported here.

• OSTI ID: 7098798
• Journal Information: Bulletin of Environmental Contamination and Toxicology; (USA), Journal Name: Bulletin of Environmental Contamination and Toxicology; (USA) Journal Issue: 2 Vol. 44:2; ISSN 0007-4861; ISSN BECTA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACRIDINES
AMINES
AMINO ACIDS
AROMATICS
AZAARENES
AZINES
BACILLUS
BACTERIA
BIODEGRADATION
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
CYSTEINE
DECOMPOSITION
DRUGS
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
ESCHERICHIA COLI
FLAVINES
GENE REGULATION
GLUTATHIONE
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
KLEBSIELLA
LYASES
MERCURY CHLORIDES
MERCURY COMPOUNDS
MERCURY HALIDES
MICROCOCCUS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC MERCURY COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
PEPTIDES
POLYPEPTIDES
PROTEINS
PROTEUS
PYRIDINES
RADIOPROTECTIVE SUBSTANCES
RIBOFLAVIN
SALMONELLA
SHIGELLA
STREPTOCOCCUS
THIOLS
VITAMIN B GROUP
VITAMINS