Stereo and regioselectivity in ''Activated'' tritium reactions
Conference
·
OSTI ID:7095249
To investigate the stereo and positional selectivity of the microwave discharge activation (MDA) method, the tritium labeling of several amino acids was undertaken. The labeling of L-valine and the diastereomeric pair L-isoleucine and L-alloisoleucine showed less than statistical labeling at the ..cap alpha..-amino C-H position mostly with retention of configuration. Labeling predominated at the single ..beta.. C-H tertiary (methyne) position. The labeling of L-valine and L-proline with and without positive charge on the ..cap alpha..-amino group resulted in large increases in specific activity (greater than 10-fold) when positive charge was removed by labeling them as their sodium carboxylate salts. Tritium NMR of L-proline labeled both as its zwitterion and sodium salt showed also large differences in the tritium distribution within the molecule. The distribution preferences in each of the charge states are suggestive of labeling by an electrophilic like tritium species(s). 16 refs., 5 tabs.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA); Pennsylvania Univ., Philadelphia (USA). Cerebrovascular Research Center; Columbia Univ., New York (USA). Coll. of Physicians and Surgeons
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 7095249
- Report Number(s):
- BNL-41770; CONF-880787-2; ON: DE89001099
- Country of Publication:
- United States
- Language:
- English
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Journal Article
·
Wed Jul 20 00:00:00 EDT 1977
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·
OSTI ID:7295139
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·
Tue Oct 06 00:00:00 EDT 1987
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·
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Deamination and decarboxylation of amino acid under the action of tritium atoms
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·
Sat Oct 31 23:00:00 EST 1987
· Sov. Radiochem. (Engl. Transl.); (United States)
·
OSTI ID:5354474
Related Subjects
550200 -- Biochemistry
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
AMINO ACIDS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL BONDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
ISOMERS
ISOTOPES
LABELLING
LEUCINE
LIGHT NUCLEI
MOLECULAR STRUCTURE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROLINE
PYRROLES
PYRROLIDINES
RADIOISOTOPES
STEREOCHEMISTRY
TRITIUM
VALINE
YEARS LIVING RADIOISOTOPES
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
AMINO ACIDS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL BONDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
ISOMERS
ISOTOPES
LABELLING
LEUCINE
LIGHT NUCLEI
MOLECULAR STRUCTURE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROLINE
PYRROLES
PYRROLIDINES
RADIOISOTOPES
STEREOCHEMISTRY
TRITIUM
VALINE
YEARS LIVING RADIOISOTOPES