Investigation of the nature of semisynthetic esterases: Final report for the period September 15, 1981 to December 31, 1986
Semisynthetic enzymes (SSE) have been prepared by chemical means. The procedure consists of perturbing the tertiary structure of a protein, adding a modifier and crosslinking with a bifunctional reagent. In the study reported herein the protein is ribonuclease (RNase), the modifiers are indole derivatives and the bifunctional reagent is glutaralydehyde. The process results in the generation of semisynthetic esterases particularly effective in catalyzing the hydrolysis of aromatic amino acid esters. The low solubility of many of the indole derivatives used as modifiers limits the yield of SSE. Five hydroxy indole carboxylic acids are particularly effective. In addition these derivatives are more soluble than many of the other commerically available indole derivatives. The crude mixture of SSE was purified by classical methods which resulted in the identification of two types of esterases. One group has a pH optimum at about pH 6 while the optimum for the other group is at 7.5. Purification on DEAE Sephadex indicates the presence of several SSE in each group each possessing its own unique substrate specificity. 20 refs., 15 figs., 14 tabs.
- Research Organization:
- Anatrace, Inc., Maumee, OH (USA)
- DOE Contract Number:
- AC02-81ER12003
- OSTI ID:
- 7094315
- Report Number(s):
- DOE/ER/12003-9; ON: DE87005780
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
Similar Records
Investigation of the nature of semisynthetic esterases. Interim progress report, September 15, 1982-April 15, 1983
Investigation of the nature of semisynthetic esterases. Progress report, September 15, 1985-September 14, 1986
Related Subjects
ESTERASES
CHEMICAL PREPARATION
RNA-ASE
STRUCTURE-ACTIVITY RELATIONSHIPS
BIOCHEMICAL REACTION KINETICS
ENZYME ACTIVITY
FRACTIONATION
GEL PERMEATION CHROMATOGRAPHY
INDOLES
MOLECULAR STRUCTURE
PH VALUE
AROMATICS
AZAARENES
AZOLES
CHROMATOGRAPHY
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROLASES
KINETICS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHODIESTERASES
PYRROLES
REACTION KINETICS
SEPARATION PROCESSES
SYNTHESIS
550200* - Biochemistry