Antibodies to the. cap alpha. -subunit of insulin receptor from eggs of immunized hens

Journal Article · Fri Nov 01 00:00:00 EST 1985 · J. Immunol.; (United States)

OSTI ID:7092576

Song, C; Yu, J; Bai, D H; Hester, P Y; Kim, K

Simple methods for the generation, purification, and assay of antibodies to the ..cap alpha..-subunit of insulin receptor from eggs of immunized hen have been described. Chicken antibodies against the ..cap alpha..-subunit inhibit insulin binding to the receptor and stimulate glucose oxidation as well as autophosphorylation of the ..beta..-subunit. Thus the properties of chicken antibodies are very similar to those of antibodies found in human autoimmune diseases and different from rabbit antibodies obtained against the same antigen.

• Research Organization: Purdue Univ., West Lafayette, IN
• OSTI ID: 7092576
• Journal Information: J. Immunol.; (United States), Vol. 135:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
PURIFICATION
INSULIN
RECEPTORS
CHICKENS
EGGS
ELECTROPHORESIS
EXTRACTION CHROMATOGRAPHY
IODINE 125
LABELLED COMPOUNDS
PHOSPHORYLATION
RADIORECEPTOR ASSAY
ANIMALS
BETA DECAY RADIOISOTOPES
BIRDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FOWL
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques