Development of monoclonal antibodies against parathyroid hormone: genetic control of the immune response to human PTH

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(85)09119-4· OSTI ID:7092566

Nussbaum, S R; Lin, C S; Potts, Jr, J T; Rosenthal, A S; Rosenblatt, M

Seventeen monocloanl antibodies against the aminoterminal portion of parathyroid hormone (PTH) were generated by using BALB/c mouse for immunization fully biologically active synthetic human PTH-(1-34) and bovine PTH-(1-84) as immunogens, monoclonal antibody methods, and a solid-phase screening assay. Isotypic analysis of these monoclonal antibodies was performed using affinity purified goat antimouse immunoglobulins specific for IgG heavy chains and ..mu..(IgM). All antibodies were IgM as evidenced by 40 times greater than background activity when 25,000 cpm of /sup 125/I-labelled goat anti-mouse IgM was used as second antibody in a radioimmunoassay.

• OSTI ID: 7092566
• Journal Information: Methods Enzymol.; (United States), Vol. 109
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PARATHORMONE
IMMUNE REACTIONS
MONOCLONAL ANTIBODIES
CATTLE
EXPERIMENTAL DATA
GOATS
IMMUNOGLOBULINS
IODINE 125
LABELLED COMPOUNDS
MICE
RADIOIMMUNOASSAY
TRACER TECHNIQUES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
DATA
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
RUMINANTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques