Assay for parathyroid hormone receptors
Journal Article
·
· Methods Enzymol.; (United States)
The paper presents methods used to identify and quantify parathyroid hormone (PTH) receptors in kidney and bone. Experimental details are provided for the preparation of radioiodinated PTH, purification of labeled PTH, and PTH binding assays using renal plasma membranes and cultured cells from embryonic chick bone cells.
- OSTI ID:
- 7092490
- Journal Information:
- Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States) Vol. 109; ISSN MENZA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIRDS
BODY
BONE CELLS
CHEMICAL REACTIONS
CHICKENS
CONNECTIVE TISSUE CELLS
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
FOWL
HALOGENATION
HORMONES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
LABELLED COMPOUNDS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANS
PARATHORMONE
PEPTIDE HORMONES
PURIFICATION
RADIOISOTOPES
RADIORECEPTOR ASSAY
REACTION KINETICS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIRDS
BODY
BONE CELLS
CHEMICAL REACTIONS
CHICKENS
CONNECTIVE TISSUE CELLS
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
FOWL
HALOGENATION
HORMONES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
LABELLED COMPOUNDS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANS
PARATHORMONE
PEPTIDE HORMONES
PURIFICATION
RADIOISOTOPES
RADIORECEPTOR ASSAY
REACTION KINETICS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES