Immunobiology of T cell responses to Mls-locus-disparate stimulator cells. III. Helper and cytolytic functions of cloned, Mls-reactive T cell lines
Mls-specific T cell clones derived by limiting dilution were tested for cytotoxic activity in a lectin-dependent /sup 51/Cr-release assay. All the T cell clones tested were cytotoxic in such an assay in apparent contrast to previous reports (1, 2). However, only those target cells sensitive to cytolysis by other L3T4a/sup +/ cytolytic T cells (3) were killed by Mls-specific T cell clones in short term /sup 51/Cr-release assays, possibly explaining this discrepancy. All the T cell clones tested were L3T4a/sup +/,Lyt-2/sup -/ and stimulated B cells from Mls strains of mice to proliferate and secrete immunoglobulin. Furthermore, lysis of innocent bystander targets was observed when the T cells were stimulated with Mls-disparate stimulator cells. These results are consistent with those obtained with L3T4a/sup -/T cells specific for protein antigen:self Ia and that express cytotoxic potential (3).
- Research Organization:
- Yale Univ. School of Medicine, New Haven, CT
- OSTI ID:
- 7092168
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:1; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CELL KILLING
CELL PROLIFERATION
CHEMICAL ANALYSIS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GLOBULINS
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LECTINS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADIO-RELEASE ANALYSIS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES