Immunobiology of T cell responses to Mls-locus-disparate stimulator cells. III. Helper and cytolytic functions of cloned, Mls-reactive T cell lines

Journal Article · Wed Jan 01 00:00:00 EST 1986 · J. Immunol.; (United States)

OSTI ID:7092168

Katz, M E; Tite, J P; Janeway, Jr, C A

Mls-specific T cell clones derived by limiting dilution were tested for cytotoxic activity in a lectin-dependent /sup 51/Cr-release assay. All the T cell clones tested were cytotoxic in such an assay in apparent contrast to previous reports (1, 2). However, only those target cells sensitive to cytolysis by other L3T4a/sup +/ cytolytic T cells (3) were killed by Mls-specific T cell clones in short term /sup 51/Cr-release assays, possibly explaining this discrepancy. All the T cell clones tested were L3T4a/sup +/,Lyt-2/sup -/ and stimulated B cells from Mls strains of mice to proliferate and secrete immunoglobulin. Furthermore, lysis of innocent bystander targets was observed when the T cells were stimulated with Mls-disparate stimulator cells. These results are consistent with those obtained with L3T4a/sup -/T cells specific for protein antigen:self Ia and that express cytotoxic potential (3).

• Research Organization: Yale Univ. School of Medicine, New Haven, CT
• OSTI ID: 7092168
• Journal Information: J. Immunol.; (United States), Vol. 136:1
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
LECTINS
CELL KILLING
CELL CULTURES
CELL PROLIFERATION
CHROMIUM 51
IMMUNOGLOBULINS
LABELLED COMPOUNDS
LYMPHOCYTES
MICE
RADIO-RELEASE ANALYSIS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
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BODY FLUIDS
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CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
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INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques