Separation of cellular and viral DNA polymerases by affinity chromatography on polynucleotide--Sepharose
Journal Article
·
· Cancer Biochem. Biophys.; (United States)
OSTI ID:7091147
Polyguanylate- and poly(2'-O-methyl)uridylate-Sepharose have been prepared for affinity chromatography of DNA polymerases of viral origin (reverse transcriptase). Both cellular DNA polymerases and reverse transcriptase bind to polyguanylate-Sepharose. The cellular polymerases can be eluted from the column between 0.32 and 0.42 M NaCl while reverse transcriptase eluted between 0.56 and 0.78 M NaCl. However, only reverse transcriptase adheres to poly(2'-O-methyl)uridylate-Sepharose and can be eluted at approximately 0.35 M NaCl. The columns were used to partially purify RNA-dependent DNA polymerase from spleens of mice infected with Rauscher leukemia virus. The enzyme preparation is about 1300-fold purified and is inhibited by antiserum prepared against purified reverse transcriptase from Rauscher leukemia virus to the same extent as the virion enzyme.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 7091147
- Journal Information:
- Cancer Biochem. Biophys.; (United States), Journal Name: Cancer Biochem. Biophys.; (United States) Vol. 1; ISSN CABCD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Radioimmunoassay for mammalian type C viral reverse transcriptase. [/sup 125/I tracer technique]
Isolation and properties of the vaccinia virus DNA-dependent RNA polymerase
RNA binding and replication by the poliovirus RNA polymerase
Journal Article
·
Sun May 01 00:00:00 EDT 1977
· J. Virol.; (United States)
·
OSTI ID:7313405
Isolation and properties of the vaccinia virus DNA-dependent RNA polymerase
Journal Article
·
Mon Oct 10 00:00:00 EDT 1977
· J. Biol. Chem.; (United States)
·
OSTI ID:7282346
RNA binding and replication by the poliovirus RNA polymerase
Thesis/Dissertation
·
Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:6622841