Correlation between UV dose requirement for lambda bacteriophage induction and lambda repressor concentration

Journal Article · Thu Jun 01 04:00:00 EDT 1978 · J. Virol.; (United States)

OSTI ID:7090724

Baluch, J; Sussman, R

Escherichia coli K-12 wild type and a uvrA mutant derivative were used to construct isogenic strains bearing one, two, three, or more phage lambda cI genomes and containing increasing concentrations of lambda repressor as measured by in vitro operator DNA-binding assays. The survival and phage induction in response to uv irradiation were determined. In both strains, dose-response relationships were obtained as a function of the cellular repressor concentration. The uvrA lysogens required one-tenth the uv fluence of the wild-type counterparts for induction. Lysogenic strains containing plasmids that overproduce the lambda ind/sup +/ repressor and the same lysogens with plasmids overproducing the lambda ind/sup -/ repressor displayed the same suvival curves as the nonlysogenic parental strain; however, only the former produced infectious centers (at a frequency of 2 x 10/sup -3/ to 5 x 10/sup -4/) in response to radiation.

• Research Organization: Univ. of Pittsburgh
• OSTI ID: 7090724
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 26:3; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
INHIBITION
IRRADIATION
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
QUANTITY RATIO
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
SURVIVAL TIME
ULTRAVIOLET RADIATION
VIRUSES