Radioimmunoimaging of pneumocystis carinii infection in rats
Pneumocystis carinil pneumonia (PCP) is seen in patients with impaired immunity due to chemotherapeutic suppression or to a primary disorder, congenital or AIDS. Although radiogallium imaging has been helpful in the workup of PCP, it is non-specific. Since there is no early specific non-invasive method to diagnose PCP, the authors are developing an imaging technique using radiolabeled antibodies. Fulminant PCP was induced in rats by injecting cortisone, 20mg 2-3 times/wk for 8 wks. PC cells isolated from rat lung were injected into rabbits. The antiserum thus derived was separated and purified using Protein-A bound sepharose column with identification of IgG by polyacrylamide gel electrophoresis. Both rabbit antipneumocystis antibodies and purified IgG(Sigma) were iodinated with I-131 to a high specific activity (3-5..mu..Ci/ug) using a lactoperoxidase method. /sup 131/I-labeled specific and non-specific IgG were injected into rats with PC infection and imaged with an Anger camera. After sacrifice, I-131 activity/gram tissue (lung, liver, heart) was determined and expressed as organ ratios. An increased uptake of specific antibody in lungs of rats with PCP was demonstrated by organ counting and imaging. This increase was not seen in normal controls or rats injected with non-specific IgG. These data provide a basis for radioimmunoimaging of infectious diseases.
- Research Organization:
- Mount Sinai Medical Center, New York, NY
- OSTI ID:
- 7090399
- Report Number(s):
- CONF-840619-; TRN: 87-008771
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 25:5; Conference: 31. annual meeting of the Society of Nuclear Medicine, Los Angeles, CA, USA, 5 Jun 1984
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANTIBODIES
DIAGNOSTIC USES
RADIOIMMUNOASSAY
IODINE 131
UPTAKE
PNEUMONIA
DIAGNOSTIC TECHNIQUES
AIDS
CORTISONE
GALLIUM ISOTOPES
IMAGE PROCESSING
INJECTION
LABELLING
LUNGS
PATIENTS
POLYAMIDES
PROTEINS
RABBITS
RATS
ADRENAL HORMONES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DISEASES
GLUCOCORTICOIDS
HEMIC DISEASES
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INFECTIOUS DISEASES
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
POLYMERS
PREGNANES
PROCESSING
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
USES
VERTEBRATES
VIRAL DISEASES
550601* - Medicine- Unsealed Radionuclides in Diagnostics