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Macromolecular photoaffinity labeling wth radioactive photoactivable heterobifunctional reagents

Journal Article · · Anal. Biochem.; (United States)
;

Three new photoactivable heterobifunctional reagents which can be radioiodinated have been synthesized. One of the radioactive reagents, the N-hydroxysuccinimide ester of 4-azidosalicylic acid was coupled to concanavalin A and the lectin derivative was used for the photoaffinity labeling of the lectin receptor on the erythrocyte ghost membrane.

Research Organization:
Univ. of Wyoming, Laramie
OSTI ID:
7089914
Journal Information:
Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 121:2; ISSN ANBCA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANTIBODIES
AZIDO COMPOUNDS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL ANALYSIS
CHEMICAL BONDS
CHEMICAL REACTIONS
COMPLEXES
CONCANAVALIN
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROCYTES
HEMAGGLUTININS
HYDROXY ACIDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LECTINS
LIGANDS
MATERIALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOCHEMICAL ANALYSIS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
SALICYLIC ACID
TRACER TECHNIQUES