Studies on the preparation of radiolabelled monoclonal antibodies

Conference · Sun Jan 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:7089428

Motta-Hennessy, C; Eccles, S; Dean, C J; Barrett, M; Ott, R J

As part of a program to evaluate techniques for radiolabelling antibodies, the authors have used human IgG as a model system to study the desferoxamine (DF) coupling reaction using glutaraldehyde or carbodiimide at various concentrations. The IgG-DF has been labelled with a Ga-67 and In-111 with a labelling efficiency of 60-96% with good reproducibility and stability. Radiochemical purity was assayed by Sephacril S-300, Sephadex-G50 or HPLC using TSK 300SW column with similar results. Labelling efficiency in relation with storage time of the conjugate was analysed by Sephadex-G50 and paper chromatography using Whatman number1 and 85% Methanol: with yields ranging from 96-34% after 1-30 days storage at 4/sup 0/C. The electrophoretic mobility was determined by SDS polyacrylamide gel electrophoresis. The conjugate was found to possess similar electrophoretic mobility as native IgG. As an extension of this work, the authors have conjugated the rat IgG/sub 2b/ MoAb M10/76 (which is specific for the chemically induced MC24 rat sarcoma) with DF at a M:R 1:40. The plasma clearance in normal rats 48 hours after injection was 4 hours versus 18-24 hours for the I-125 labelled M10/76. Localization of the antibody into the MC24 tumour in syngeneic Lister hooded rats is being evaluated and compared with a similar antibody (11/160) with specificity for the rat sarcome HSN/sub t.c./ The rapid blood clearance of this rat MoAb precludes high concentrations in the tumour and enables imaging within a shorter time after injection.

• Research Organization: Royal Marsden Hospital, Sutton, Surrey
• OSTI ID: 7089428
• Report Number(s): CONF-840619-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 25:5
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALCOHOLS
ALDEHYDES
AMINES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC USES
DISEASES
EFFICIENCY
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
GALLIUM 67
GALLIUM ISOTOPES
GEL PERMEATION CHROMATOGRAPHY
HYDROXY COMPOUNDS
IMIDES
IMPURITIES
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METHANOL
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC POLYMERS
POLYAMIDES
POLYMERS
QUANTITY RATIO
RADIOISOTOPES
RATS
RODENTS
SARCOMAS
SEPARATION PROCESSES
TRACER TECHNIQUES
USES
VERTEBRATES