Two thymidylate synthetases in Bacillus subtilis (thymine-requiring mutants/trimethoprim/dUMP pools)
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
Bacillus subtilis grown at temperatures below 37/sup 0/ contains two thymidylate synthetases, TSaseA and TSaseB. Their presence is dependent on functional thyA and thyB genes, respectively. When cells are grown at 46/sup 0/ they only contain TSaseA activity. This allows an easy positive selection for thyA and thyA, thyB mutants. The two TSases have been physically separated, and they show similar overall requirements for activity. However, they differ significantly in both their kinetic and their physicochemical properties.
- Research Organization:
- Univ. Institute of Biological Chemistry, Copenhagen, Denmark
- OSTI ID:
- 7089264
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 75:3; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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