Relative rates of sugar utilization by an ethanologenic recombinant Escherichia coli using mixtures of glucose, mannose, and xylose

Journal Article · · Applied Biochemistry and Biotechnology; (United States)

DOI:https://doi.org/10.1007/BF02941812· OSTI ID:7086304

Lawford, H G; Rousseau, J D ^[1]

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1. Univ. of Toronto, Ontario (Canada)

The volumetric rates of glucose (G), mannose (M), and xylose (X) utilization by recombinant Escherichia coli B (pLO1297) were compared in pH-stat batch fermentations with Luria broth containing various combinations of two of these sugars at differing mass ratios. Using single substrate media, the rates of glucose, mannose, and xylose utilization were 3.0, 0.8, and 1.5 g/L/h, respectively. With all two-substrate media, hexose and pentose sugars were consumed simultaneously. At a mass ratio of 2:1 (M or X:G), the rate of glucose utilization was reduced to 1.7 and 1.2 g/L/h by mannose and xylose, respectively. In media containing glucose and xylose, the rate of xylose utilization was inhibited when the glucose component exceeded about 40% of the total sugar mass in the medium. At a mass ratio of 2M:1X, mannose did not inhibit the rate of xylose utilization. At a mass ratio of 1:2 (G or X:M), the rate of mannose utilization was unaffected by either glucose or xylose. 38 refs., 5 figs., 1 tab.

• OSTI ID: 7086304
• Journal Information: Applied Biochemistry and Biotechnology; (United States), Vol. 45-46; ISSN 0273-2289
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
BIOMASS
BIOCONVERSION
ESCHERICHIA COLI
BIOLOGICAL PATHWAYS
GLUCOSE
METABOLISM
MANNOSE
XYLOSE
ALDEHYDES
BACTERIA
CARBOHYDRATES
ENERGY SOURCES
HEXOSES
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
RENEWABLE ENERGY SOURCES
SACCHARIDES
090900* - Biomass Fuels- Processing- (1990-)
550700 - Microbiology