Effects of /sup 60/Co gamma irradiation on the polymerization of microtubules in vitro
Journal Article
·
· Radiat. Res.; (United States)
Microtubules have been shown to depolymerize in vitro into a high-molecular-weight aggregate and a 6S component. Irradation of these components separately has indicated that the 6S component is preferentially damaged. This was shown by a reduction in the extent of polymerization following reconstitution of the irradiated and control fractions. The irradiation had no apparent effect on either protein kinase activity or on (/sup 14/C)-colchicine binding ability. In addition, irradiation of depolymerized microtubules at various protein concentrations has demonstrated that the 6S tubulin is also preferentially damaged in the presence of the high-molecular-weight component.
- Research Organization:
- Georgetown, Univ., Washington, DC
- OSTI ID:
- 7083217
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 71:2; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
IN VITRO
IONIZING RADIATIONS
KIDNEYS
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEINS
RADIATION EFFECTS
RADIATIONS
TUBULES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
IN VITRO
IONIZING RADIATIONS
KIDNEYS
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEINS
RADIATION EFFECTS
RADIATIONS
TUBULES