Cloning of the human dopamine D5 receptor gene and identification of a highly polymorphic microsatellite for the DRD5 locus that shows tight linkage to the chromosome 4p reference marker RAF1P1

Journal Article · Mon Nov 01 00:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1489· OSTI ID:7077696

Sherrington, R; Mankoo, B; Kalsi, G; Gurling, H; Curtis, D ^[1]; Attwood, J; Povey, S ^[2]; Buetow, K ^[3]

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1. Univ. College London Medical School (United Kingdom)
2. Galton Lab., London (United Kingdom)
3. Fox Chase Cancer Center, Philadelphia, PA (United States)

The authors identified a cosmid clone with exact sequence homology to part of the human dopamine D5 receptor gene (DRD5) after screening a cosmid library with the human DRD1 gene. The dopamine D5 receptor was mapped to chromosome 4p15.1-p15.3 by in situ hybridization and using a somatic cell hybrid panel. They report here the further localization of the DRD5 gene following identification of a highly polymorphic dinucleotide repeat sequence in the cosmid clone. The microsatellite (D5(CT/GT/GA)[sub n]) had 12 alleles with a polymorphic information content value of 0.77. Linkage analysis in 39 CEPH pedigrees demonstrated tight linkage to the chromosome 4p reference marker RAF1P1 (Z[sub maxf] 20.66 at [theta][sub f] 0.05 and Z[sub maxM] 16.57 at [theta][sub m] 0.07). 16 refs., 2 tabs.

• OSTI ID: 7077696
• Journal Information: Genomics; (United States), Vol. 18:2; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DOPAMINE
RECEPTORS
HUMAN CHROMOSOMES
GENETIC MAPPING
GENES
DNA-CLONING
AMINES
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DRUGS
HYBRIDIZATION
HYDROXY COMPOUNDS
MAPPING
MEMBRANE PROTEINS
NEUROREGULATORS
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
PROTEINS
SYMPATHOMIMETICS
550400* - Genetics