Primary structure of the human fgr proto-oncogene product p55/sup c-fgr/
Normal human c-fgr cDNA clones were constructed by using normal peripheral blood mononuclear cell mRNA as a template. Nucleotide sequence analysis of two such clones revealed a 1,587-base-pair-long open reading frame which predicted the primary amino acid sequence of the c-fgr translational product. Homology of this protein with the v-fgr translational product stretched from codons 128 to 516, where 32 differences among 388 codons were observed. Sequence similarity with human c-src, c-yes, and fyn translations products began at amino acid position 76 of the predicted c-fgr protein and extended nearly to its C-terminus. In contrast, the stretch of 75 amino acids at the N-terminus demonstrated a greatly reduced degree of relatedness to these same proteins. To verify the deduced amino acid sequence, antibodies were prepared against peptides representing amino- and carboxy-terminal regions of the predicted c-fgr translational product. Both antibodies specifically recognized a 55-kilodalton protein expressed in COS-1 cells transfected with a c-fgr cDNA expression plasmid. Moreover, the same protein was immunoprecipitated from an Epstein-Barr virus-infected Burkitt's lymphoma cell line which expressed c-fgr mRNA but not in its uninfected fgr mRNA-negative counterpart. These findings identified the 55-kilodalton protein as the product of the human fgr proto-oncogene.
- Research Organization:
- Lab. of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD (US)
- OSTI ID:
- 7077510
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:1; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CLONING
DISEASES
DNA SEQUENCING
DNA-CLONING
GENES
GENETIC MAPPING
IMMUNE SYSTEM DISEASES
IMMUNOASSAY
LEUKOCYTES
LYMPHOMAS
MAMMALS
MAN
MAPPING
MATERIALS
MESSENGER-RNA
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
MONOCYTES
NEOPLASMS
NUCLEIC ACIDS
NUCLEOPROTEINS
ONCOGENES
ORGANIC COMPOUNDS
PHENOTYPE
PRIMATES
PROTEINS
RNA
STRUCTURAL CHEMICAL ANALYSIS
STRUCTURE-ACTIVITY RELATIONSHIPS
VERTEBRATES