Construction of a yeast artificial chromosome contig spanning the pseudoautosomal region and isolation of 25 new sequence-tagged sites
- Institut Pasteur, Paris (France) Laboratoire de Cytogenetique et Genetique Oncologiques, Villejuif (France)
- Centre d'Etude du Polymorphisme Humain, Paris (France)
- Unite de Genetique Moleculaire Humaine, Institut Pasteur, Paris (France); and others
Thirty-one yeast artificial chromosomes (YACs) from the human pseudoautosomal region were identified by a combination of sequence-tagged site (STS) screenings and colony hybridizations, using a subtelomeric interspersed repetitive element mapping predominantly to the pseudoautosomal region. Twenty-five new pseudoautosomal STSs were generated, of which 4 detected restriction fragment length polymorphisms. A total of 33 STSs were used to assemble the 31 YACs into a single contiguous set of overlapping DNA fragments spanning at least 2.3 megabases of the pseudoautosomal region. In addition, four pseudoautosomal genes including hydroxyindole O-methyltransferase have been positioned on this set of fragments. 48 refs., 1 fig., 3 tabs.
- OSTI ID:
- 7077445
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 16:3; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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