Evidence that the SRY protein is encoded by a single exon on the human Y chromosome
- Massachusetts Institute of Technology, Cambridge, MA (United States) Women's Hospital and Harvard Medical School, Boston, MA (United States)
- Massachusetts Institute of Technology, Cambridge, MA (United States)
To facilitate studies of the SRY gene, a 4741-bp portion of the sex-determining region of the human Y chromosome was sequenced and characterized. Two RNAs were found to hybridize to this genomic segment, one transcript deriving from SRY and the second cross-hybridizing to a pseudogene located 2.5 kb 5[prime] of the SRY open reading frame (ORF). Analysis of the SRY transcript using 3[prime] and 5[prime] rapid amplification and cloning of ends suggested that the entire SRY protein is encoded by a single exon. A 700-bp CpG island is located immediately 5[prime] of the pseudogene (and 2 kb 5[prime] of the SRY ORF). Within this CpG island lies the sequence CGCCCCCGC, a potential binding site for the EGR-1/WT1 family of transcription factors, some of which appear to function in gonadal development. 19 refs., 1 fig.
- OSTI ID:
- 7077366
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 17:3; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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