Interactions of 4-nitroquinoline 1-oxide with four deoxyribonucleotides

Journal Article · Mon Apr 03 23:00:00 EST 1978 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00600a033· OSTI ID:7076103

Winkle, S A; Tinoco, I Jr

The interactions of 4-nitroquinoline 1-oxide (NQO) with the four 5'-deoxyribonucleotides were probed using absorption spectra of the charge transfer bands and /sup 1/H and /sup 13/C nuclear magnetic resonance (NMR) spectra of nucleotide-NQO mixtures. Spectral data yielded equilibrium constants (K(dpG:NQO) = 16 M/sup -1/, K(dpA:NQO) = 12 M/sup -1/, K(dpT:NQO) = K(dpC:NQO) = 4 M/sup -1/) which suggest the preference of NQO for the guanine residue in a DNA. This is in agreement with the data of Okano, T., et al. ((1969) Gann 60, 295). From /sup 13/C and /sup 1/H NMR data on nucleosides, a structure for the dpG:NQO complex is proposed.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 7076103
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 17:7; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTRA
AZINES
BIOCHEMICAL REACTION KINETICS
DNA
HETEROCYCLIC COMPOUNDS
KINETICS
MAGNETIC RESONANCE
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIDINES
QUINOLINES
REACTION KINETICS
RESONANCE
SPECTRA