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Inactivation of cathepsin B/sub 1/ by diazomethyl ketones

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)

Benzyloxycarbonyl-phenylalanyl diazomethyl ketone and benzyloxy-carbonyl-phenylalanyl-phenylalanyl diazomethyl ketone, which have been shown to inactivate the thiol protease papain by a mechanism different from that of substrate chloromethyl ketone derivatives, have now been examined as inhibitors of cathepsin B/sub 1/ of beef spleen. The dipeptide derivative irreversibly inactivates this protease rapidly, apparently by affinity labeling.

Research Organization:
Brookhaven National Lab., Upton, NY
OSTI ID:
7076091
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 79:3; ISSN BBRCA
Country of Publication:
United States
Language:
English

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