Biomonitoring of nitropolynuclear aromatic hydrocarbons via protein and DNA adducts. Research report, August 1987-July 1991

Technical Report · Sat Jan 01 04:00:00 EST 1994

OSTI ID:7074923

El-Bayoumy, K; Johnson, B E; Roy, A K; Upadhyaya, P; Partian, S J

The authors developed methods to detect and quantify adducts of 1-nitropyrene and 1,6-dinitropyrene with proteins and with DNA. ((3)H)-Nitropyrene given to male or female F344 or Sprague-Dawley rats by gavage at 5 dose levels (0.1 to 1000 ug/kg) led to stable hemoglobin adducts, which accounted for 0.08% + or - 0.005% of the dose. The radioactivity was associated with the heme moiety rather than the globin and was cleared with a half-life of 13.6 days ((3)H)1,6-dintropyrene also bound to rat hemoglobin at a level that is 0.04% of the dose given by gavage and was bound to the heme moiety. Comparing the rather complex DNA adduct pattern formed with 1-nitropyrene to the simple adduct pattern formed with 1,6-dinitropyrene in the rat suggests that N-(deoxyguanosin-8-yl)-1-amino-6-nitropyrene, derived from 1,6-dinitropyrene, may be a better dosimeter, even though 1,6-dinitropyrene is present in environment in far lower concentrations than 1-nitropyrene.

• Research Organization: American Health Foundation, Inc., Valhalla, NY (United States). Div. of Chemical Carcinogenesis
• OSTI ID: 7074923
• Report Number(s): PB-94-182821/XAB; CNN: HEI-RFA-86-2
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540120 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (1990-)
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AIR POLLUTION
AROMATICS
BIOASSAY
BIOLOGICAL INDICATORS
DNA ADDUCTS
HYDROCARBONS
ORGANIC COMPOUNDS
POLLUTION
POLYCYCLIC AROMATIC HYDROCARBONS